ICAM5 Polyclonal antibody

• 

  WB analysis of mouse brain using 12759-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12759-1-AP (ICAM5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12759-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12759-1-AP (ICAM5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ICAM5 Polyclonal Antibody for WB, ELISA

Cat No. 12759-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ICAM 5, ICAM5, Telencephalin, TLCN, TLN

---

Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12759-1-AP targets ICAM5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3303

  Product name: Recombinant human ICAM5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 34-355 aa of BC026338

  Sequence: FWADLQPRVAFVERGGSLWLNCSTNCPRPERGGLETSLRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELMPLPPWQPVGENFTLSCRVPGAGPRASLTLTLLRGAQELIRRSFAGEPPRARGAVLTATVLARREDHGANFSCRAELDLRPHGLGLFENSSAPRELRTFSLSPDAPRLAAPRLLEVGSERPVSCTLDGLFPASEARVYLALGDQNLSPDVTLEGDAFVATATATASAEQEGARQLICNVTLGGENRETRENVTIYSFPAPLLTLSEPSVSEGQMVTVTCAAGTQALVTLE

• Full Name: intercellular adhesion molecule 5, telencephalin
• Calculated Molecular Weight: 924 aa, 97 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC026338
• Gene Symbol: ICAM5
• Gene ID (NCBI): 7087
• RRID: AB_10644149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UMF0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM-5, also known as telencephalin, is a type I integral membrane glycoprotein of the immunoglobulin superfamily. It is specifically expressed in the postnatal excitatory neuronal cell bodies, dendritic shafts, and dendritic filopodia of the telencephalon. ICAM-5 functions in both the immune and nervous systems. ICAM-5 strongly stimulates neurite outgrowth. It is cleaved by matrix metalloproteases upon activation of glutamate receptors or degraded through endocytosis resulting in increased spine maturation. The cleaved soluble fragment of ICAM-5 is immunosuppressive, which may be important in neuronal inflammatory diseases. (PMID: 10402269; 17682049; 25300135)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncotarget; Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein.; Authors - Yanxiao Zhang