Tested Applications
| Positive WB detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17511-1-AP targets ICMT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | isoprenylcysteine carboxyl methyltransferase |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32-34 kDa |
| GenBank Accession Number | NM_012405 |
| Gene Symbol | ICMT |
| Gene ID (NCBI) | 23463 |
| RRID | AB_2293346 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60725 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ICMT antibody 17511-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ICMT polyclonal antibody (Cat# 17511-1-AP) has 1 citation. It was published in Cell Communication and Signaling (IF 11.6), a study investigating how ICMT deficiency affects weight loss, mortality, and tumor formation in a mouse model of liver cancer. The antibody was used for Western blot in mouse samples. The research field is hepatocellular carcinoma/liver cancer biology.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal ICMT deficiency ameliorates weight loss and mortality, but not tumor formation in a mouse model of liver cancer.
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