ICOSLG Recombinant monoclonal antibody, PBS Only (Detector)

• 

  Sandwich ELISA sample test of MP01890-1

  Serum of six individual healthy human donors was measured. The human ICOSLG/B7-H2/CD275 concentration of detected samples was determined to be 16.96 ng/mL with a range of 12.81 - 29.75 ng/mL.

• 

  Sandwich ELISA sample test of MP01890-1

  The mean ICOSLG/B7-H2/CD275 concentration was determined to be 63.95 ng/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP01890-1

  Sandwich ELISA standard curve of MP01890-1, Human ICOSLG/B7-H2/CD275 Recombinant Matched Antibody Pair - PBS only. 85173-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0008. 85173-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

• 

  Cytometric bead array standard curve of MP01890-1

  Cytometric bead array standard curve of MP01890-1, ICOSLG/B7-H2/CD275 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85173-3-PBS. Detection antibody: 85173-1-PBS. Standard: Eg0008. Range: 1.563-200 ng/mL.

• 

  Affinity and Kinetic Characterization of 85173-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 85173-1-PBS against Human ICOSLG was performed. The affinity constant is 1.26 nM.

ICOSLG Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 85173-1-PBS

Clone No.242241B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

B7H2, B7 H2, B7-like protein Gl50, B7-related protein 1, B7RP1

---

Product Information

85173-1-PBS targets ICOSLG as part of a matched antibody pair:

MP01890-1: 85173-3-PBS capture and 85173-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0008

  Product name: Recombinant Human ICOSLG/B7-H2/CD275 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 19-256 aa of BC064637

  Sequence: DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAAT

• Full Name: inducible T-cell co-stimulator ligand
• Calculated Molecular Weight: 33 kDa, 34 kDa
• GenBank Accession Number: BC064637
• Gene Symbol: ICOSLG
• Gene ID (NCBI): 23308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75144
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.