Anti-Human ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody

• 

  FC experiment of human monocyte-derived mature dendritic cells using 98358-1-RR

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human ICOSLG/B7-H2/CD275 Rabbit RecAb (98358-1-RR, Clone: 242241C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98358-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98358-1-RR against Human ICOSLG/B7-H2/CD275 were performed. The affinity constant is 10.4 nM.

ICOSLG/B7-H2/CD275 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98358-1-RR

Clone No.242241C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

B7H2, ICOSLG, B7 H2, B7-like protein Gl50, B7-related protein 1

---

Tested Applications

• Positive FC detected in: human monocyte-derived mature dendritic cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98358-1-RR targets ICOSLG/B7-H2/CD275 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ICOSLG/B7-H2/CD275 fusion protein Eg0008
• Full Name: inducible T-cell co-stimulator ligand
• Calculated Molecular Weight: 33 kDa, 34 kDa
• GenBank Accession Number: BC064637
• Gene Symbol: ICOSLG
• Gene ID (NCBI): 23308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75144
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).