ICT1 Polyclonal antibody

ICT1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10403-1-AP

Synonyms

MRPL58, Large ribosomal subunit protein mL62, EC:3.1.1.29, DS-1, DS1

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Validation Data Gallery

• 

  WB analysis of A2780 using 10403-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10403-1-AP (ICT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10403-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10403-1-AP (ICT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SKOV-3 using 10403-1-AP

  IP result of anti-ICT1 (IP:10403-1-AP, 4ug; Detection:10403-1-AP 1:300) with SKOV-3 cells lysate 2000ug.

• 

  IF Staining of HeLa using 10403-1-AP

  Immunofluorescent analysis of HeLa cells using 10403-1-AP (ICT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A2780 cells, SKOV-3 cells
• Positive IP detected in: SKOV-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

10403-1-AP targets ICT1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ICT1 fusion protein Ag0647
• Full Name: immature colon carcinoma transcript 1
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 21-24 kDa
• GenBank Accession Number: BC015335
• Gene Symbol: ICT1
• Gene ID (NCBI): 3396
• RRID: AB_2122853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells. This antibody is a rabbit polyclonal antibody raised against a full-length human ICT1 protein.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Cell Rep; The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly.; Authors - Ya-Ting Tu
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
human	WB	PLoS Genet; RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.; Authors - Aurelio Reyes
human	WB	Mitochondrion; Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.; Authors - Dujeancourt Laurent L
human	WB	Biochem J; The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome.; Authors - Agata Rozanska