ID3 Polyclonal antibody

• Featured Product
• KD/KO Validated

ID3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10389-1-AP

Synonyms

1R21, bHLHb25, HEIR 1, HEIR1, ID3, Inhibitor of DNA binding 3

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Validation Data Gallery

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  WB analysis of Transfected HEK-293 using 10389-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10389-1-AP (ID3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10389-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10389-1-AP (ID3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

10389-1-AP targets ID3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, mink
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ID3 fusion protein Ag0588
• Full Name: inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
• Calculated Molecular Weight: 119 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC003107
• Gene Symbol: ID3
• Gene ID (NCBI): 3399
• RRID: AB_2248830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3, synonym: HEIR-1) is a member of the ID family of HLH proteins, which lacks a basic DNA-binding domain and inhibits transcription through formation of nonfunctional dimers that are incapable of binding to DNA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Bcl-3 regulates TGFβ signaling by stabilizing Smad3 during breast cancer pulmonary metastasis.; Authors - Xi Chen
mouse	WB	Biomedicines; BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during Aging.; Authors - Tingting Li
rat	IHC	Cancer Sci; Cellular distributions of molecules with altered expression specific to thyroid proliferative lesions developing in a rat thyroid carcinogenesis model.; Authors - Woo Gye-Hyeong GH
mink	WB	Life Sci; Pantothenic acid promotes dermal papilla cell proliferation in hair follicles of American minks via inhibitor of DNA Binding 3/Notch signaling pathway.; Authors - Zhuo Wang ; KD Validated