IDH3G Polyclonal antibody

• Home
• Products
• Primary Antibodies
• IDH3G Polyclonal antibody (25848-1-AP)

IDH3G Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25848-1-AP Copied!

Publications (2)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

NAD(+)-specific ICDH subunit gamma, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H IDHG

• 

  WB analysis of mouse colon using 25848-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 25848-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A375 using 25848-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for IDH3G in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: mouse colon tissue, A375 cells, A431 cells
• Positive IHC detected in: human colon tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25848-1-AP targets IDH3G in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23113

  Product name: Recombinant human IDH3G protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-393 aa of BC000933

  Sequence: MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVINGRAVEA

• Full Name: isocitrate dehydrogenase 3 (NAD+) gamma
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC000933
• Gene Symbol: IDH3G
• Gene ID (NCBI): 3421
• RRID: AB_2880267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51553
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The IDH3G Polyclonal antibody (Cat# 25848-1-AP) has 2 total citations. These appear in Cell Reports in Medicine and Oncogene. The cited research spans neuroscience (peripheral nerve injury and neuronal mitochondrial metabolism) and oncology (hypoxia-driven tricarboxylic acid cycle reprogramming in breast tumorigenesis). Both studies utilized the antibody for Western blot applications, with one in mouse and one in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Rep Med; Acutely denervated muscle EVs reshape neuronal mitochondrial metabolism via retrograde signaling to rescue peripheral nerve injury.; Authors - Qi Liu
human	WB	Oncogene; Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.; Authors - Ke Tang