IER2 Polyclonal antibody

IER2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23849-1-AP

Synonyms

3CH92, CHX1, ETR101, IER2, immediate early response 2, Pip92, Protein ETR101

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Validation Data Gallery

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  IHC staining of human liver using 23849-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23849-1-AP (IER2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23849-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23849-1-AP (IER2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 23849-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23849-1-AP (IER2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 23849-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23849-1-AP (IER2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 23849-1-AP is IER2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IER2 fusion protein Ag20869
• Full Name: immediate early response 2
• Calculated Molecular Weight: 223 aa, 24 kDa
• GenBank Accession Number: BC072432
• Gene Symbol: IER2
• Gene ID (NCBI): 9592
• RRID: AB_2879339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ier2 (also called ETR101, Pip92, 3CH92 and CHX1, hereafter referred to as Ier2, regardless of species) is an immediate early gene (PMID: 2678945) that was originally found to be upregulated in fibroblasts upon their activation (PMID: 3841511). Immediate early genes are a group of genes that are rapidly induced in quiescent cells by proliferation- and migration-inducing stimuli. Ier2 may have a role in tumor cell motility and metastasis (PMID: 22120713).

Publications

Species	Application	Title
human	WB	Cell Signal; IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A.; Authors - Takumi Ueda
human	WB	FEBS J; PP2A-B55 and its adapter proteins IER2 and IER5 regulate the activity of RB family proteins and the expression of cell cycle-related genes; Authors - Kuriko Doi