IFIT1L Polyclonal antibody

IFIT1L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21483-1-AP

Synonyms

IFIT1B, IFIT1L

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 21483-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 21483-1-AP (IFIT1L Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of mouse testis using 21483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21483-1-AP (IFIT1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21483-1-AP (IFIT1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 21483-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21483-1-AP (IFIT1L Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 21483-1-AP

  Immunofluorescent analysis of HEK-293 cells using 21483-1-AP (IFIT1L antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of RAW 264.7 using 21483-1-AP

  Immunofluorescent analysis of RAW 264.7 cells using 21483-1-AP (IFIT1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IHC detected in: mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21483-1-AP targets IFIT1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFIT1L fusion protein Ag15765
• Full Name: interferon-induced protein with tetratricopeptide repeats 1-like
• Calculated Molecular Weight: 474 aa, 55 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC150189
• Gene Symbol: IFIT1L
• Gene ID (NCBI): 439996
• RRID: AB_2878867
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.