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  • KD/KO Validated

IFIT3 Antibody

IFIT3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15201-1-AP

Synonyms

CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15201-1-AP targets IFIT3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, Monkey, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIT3 fusion protein Ag7349
Full Name interferon-induced protein with tetratricopeptide repeats 3
Calculated molecular weight 56 kDa
Observed molecular weight 60 kDa
GenBank accession number BC004977
Gene symbol IFIT3
Gene ID (NCBI) 3437
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa interferon (IFN) induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

Protocols

Product Specific Protocols
WB protocol for IFIT3 antibody 15201-1-APDownload protocol
IHC protocol for IFIT3 antibody 15201-1-APDownload protocol
IF protocol for IFIT3 antibody 15201-1-APDownload protocol
IP protocol for IFIT3 antibody 15201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Metab

Mapping the molecular signatures of diet-induced NASH and its regulation by the hepatokine Tsukushi.

Authors - Xuelian Xiong
MonkeyWB

Antiviral Res

Poly(I:C) inhibits porcine reproductive and respiratory syndrome virus replication in MARC-145 cells via activation of IFIT3.

Authors - Zhang Lili L
  • KD Validated
mouseWB

Proteomics

Proteomic analysis of the lungs of mice infected with different pathotypes of H5N1 avian influenza viruses.

Authors - Zhao Dongming D
humanWB

Mol Cancer Res

STAT1 and NF-κB Inhibitors Diminish Basal Interferon-Stimulated Gene Expression and Improve the Productive Infection of Oncolytic HSV in MPNST Cells.

Authors - Joshua D Jackson
humanWB

J Virol

Herpes Simplex Virus 1 Tegument Protein UL41 Counteracts IFIT3 Antiviral Innate Immunity.

Authors - Zhangtao Jiang
pigWB,IF

J Proteome Res

Two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproductive and respiratory syndrome virus.

Authors - Lu Qi Q