|Positive WB detected in||HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15201-1-AP targets IFIT3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, Monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFIT3 fusion protein Ag7349|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC004977|
|Gene ID (NCBI)||3437|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.
|Product Specific Protocols|
|WB protocol for IFIT3 antibody 15201-1-AP||Download protocol|
|IHC protocol for IFIT3 antibody 15201-1-AP||Download protocol|
|IF protocol for IFIT3 antibody 15201-1-AP||Download protocol|
|IP protocol for IFIT3 antibody 15201-1-AP||Download protocol|
|Click here to view our Standard Protocols|
MAVS-MKK7-JNK2 Defines a Novel Apoptotic Signaling Pathway during Viral Infection.
Progression of pathology in PINK1-deficient mouse brain from splicing via ubiquitination, ER stress, and mitophagy changes to neuroinflammation.
IFN-induced TPR protein IFIT3 potentiates antiviral signaling by bridging MAVS and TBK1.
J Proteome Res
Two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproductive and respiratory syndrome virus.
COL8A1 Promotes NSCLC Progression Through IFIT1/IFIT3-Mediated EGFR Activation.
Herpes Simplex Virus 1 Tegument Protein UL41 Counteracts IFIT3 Antiviral Innate Immunity.