IFIT3 Polyclonal antibody

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IFIT3 Polyclonal Antibody for WB, IHC-Autostainer, IP, ELISA

Cat No. 15201-1-AP Copied!

Publications (67)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC-Autostainer, IF, IP, CoIP, ChIP, ELISA

Synonyms

CIG 49, CIG49, CIG-49, GARG 49, GARG49

• 

  WB analysis using 15201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15201-1-AP (IFIT3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MKN-45 using 15201-1-AP

  IP result of anti-IFIT3 (IP:15201-1-AP, 4ug; Detection:15201-1-AP 1:4000) with MKN-45 cells lysate 1560 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 15201-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15201-1-AP (IFIT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MKN-45 cells, SGC-7901 cells
• Positive IP detected in: MKN-45 cells
• Positive IHC-Autostainer detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15201-1-AP targets IFIT3 in WB, IHC-Autostainer, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7349

  Product name: Recombinant human IFIT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 141-490 aa of BC004977

  Sequence: EEGWTQLKCGRNERAKVCFEKALEEKPNNPEFSSGLAIAMYHLDNHPEKQFSTDVLKQAIELSPDNQYVKVLLGLKLQKMNKEAEGEQFVEEALEKSPCQTDVLRSAAKFYRRKGDLDKAIELFQRVLESTPNNGYLYHQIGCCYKAKVRQMQNTGESEASGNKEMIEALKQYAMDYSNKALEKGLNPLNAYSDLAEFLETECYQTPFNKEVPDAEKQQSHQRYCNLQKYNGKSEDTAVQHGLEGLSISKKSTDKEEIKDQPQNVSENLLPQNAPNYWYLQGLIHKQNGDLLQAAKCYEKELGRLLRDAPSGIGSIFLSASELEDGSEEMGQGAVSSSPRELLSNSEQLN

• Full Name: IFIT3
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC004977
• Gene Symbol: IFIT3
• Gene ID (NCBI): 3437
• RRID: AB_2248738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14879
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

Publications

What published studies show

The IFIT3 Polyclonal antibody (Cat# 15201-1-AP) has 67 citations. These appear in journals including Signal Transduction and Targeted Therapy, Nature Biotechnology, Nature Immunology, Nature Communications, Science Translational Medicine, Advanced Science, Cell Reports, Cell Death & Disease, Cancer Letters, Journal of Neuroinflammation, EMBO Molecular Medicine, PLoS Pathogens, Journal of Virology, Journal of Immunology, Frontiers in Immunology, and bioRxiv. Research fields span antiviral innate immunity and interferon signaling, virology (influenza, SARS-CoV-2, PRRSV, HSV, HIV), oncology, neuroinflammation, and autoimmune diseases, mainly using WB, IF, and IHC.

Automatically generated from the 67 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
mouse	IF	Nat Biotechnol; DestVI identifies continuums of cell types in spatial transcriptomics data; Authors - Romain Lopez
mouse	IF	Nat Immunol; Single-cell atlas of human liver and blood immune cells across fatty liver disease stages reveals distinct signatures linked to liver dysfunction and fibrogenesis; Authors - Owen P Martin
		Nat Commun; USP24 is a cross-reactive DUB targeting MOV10 to regulate IFN-I production.; Authors - Rishov Mukhopadhyay
mouse	IF	Sci Transl Med; Hepatocytes demarcated by EphB2 contribute to the progression of nonalcoholic steatohepatitis; Authors - Yang Xiao
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia