IFIT5 Recombinant monoclonal antibody

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• IFIT5 Recombinant monoclonal antibody (86924-1-RR)

• 

  WB analysis using 86924-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86924-1-RR (IFIT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IFIT5 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86924-1-RR

Clone No.251258D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IFIT 5, IFIT-5, Interferon-induced 58 kDa protein, Interferon-induced protein with tetratricopeptide repeats 5, ISG58

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Tested Applications

• Positive WB detected in: A375 cells, PC-3 cells, HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86924-1-RR targets IFIT5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4202

  Product name: Recombinant human IFIT5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 133-482 aa of BC025786

  Sequence: YKLECPETDCEKGWALLKFGGKYYQKAKAAFEKALEVEPDNPEFNIGYAITVYRLDDSDREGSVKSFSLGPLRKAVTLNPDNSYIKVFLALKLQDVHAEAEGEKYIEEILDQISSQPYVLRYAAKFYRRKNSWNKALELLKKALEVTPTSSFLHHQMGLCYRAQMIQIKKATHNRPKGKDKLKVDELISSAIFHFKAAMERDSMFAFAYTDLANMYAEGGQYSNAEDIFRKALRLENITDDHKHQIHYHYGRFQEFHRKSENTAIHHYLEALKVKDRSPLRTKLTSALKKLSTKRLCHNALDVQSLSALGFVYKLEGEKRQAAEYYEKAQKIDPENAEFLTALCELRLSI

• Full Name: interferon-induced protein with tetratricopeptide repeats 5
• Calculated Molecular Weight: 482 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC025786
• Gene Symbol: IFIT5
• Gene ID (NCBI): 24138
• RRID: AB_3745208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13325
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.