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  • KD/KO Validated

IFITM1 Polyclonal antibody

IFITM1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11727-3-AP

Synonyms

9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inROS1728 cells
Positive IHC detected inhuman lymphoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11727-3-AP targets IFITM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, goat, African green monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFITM1 fusion protein Ag2320
Full Name interferon induced transmembrane protein 1 (9-27)
Calculated molecular weight 14 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC000897
Gene symbol IFITM1
Gene ID (NCBI) 8519
RRIDAB_2122083
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.

Protocols

Product Specific Protocols
WB protocol for IFITM1 antibody 11727-3-APDownload protocol
IHC protocol for IFITM1 antibody 11727-3-APDownload protocol
IP protocol for IFITM1 antibody 11727-3-APDownload protocol
FC protocol for IFITM1 antibody 11727-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Mitotic progression following DNA damage enables pattern recognition within micronuclei.

Authors - Shane M Harding
humanFC

Nature

Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells.

Authors - Edward J Grow
  • KD Validated
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
humanWB

Mol Ther

Type 1 Interferon Responses Underlie Tumor-Selective Replication of Oncolytic Measles Virus.

Authors - Sarah Aref
humanWB

Nat Commun

IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses

Authors - M. Clement
humanWB

Cell Rep

IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.

Authors - Jingyou Yu
  • KD Validated