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IFITM1 Antibody

IFITM1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11727-3-AP

Synonyms

9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inROS1728 cells
Positive IHC detected inhuman lymphoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11727-3-AP targets IFITM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity goat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFITM1 fusion protein Ag2320
Full Name interferon induced transmembrane protein 1 (9-27)
Calculated molecular weight 14 kDa
Observed molecular weight 14-17 kDa
GenBank accession number BC000897
Gene symbol IFITM1
Gene ID (NCBI) 8519
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.

Protocols

Product Specific Protocols
WB protocol for IFITM1 antibody 11727-3-APDownload protocol
IHC protocol for IFITM1 antibody 11727-3-APDownload protocol
IP protocol for IFITM1 antibody 11727-3-APDownload protocol
FC protocol for IFITM1 antibody 11727-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Hepatology

IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection.

Authors - Florian Wrensch
humanWB

J Infect Dis

Interferon-Induced Transmembrane Protein-Mediated Inhibition of Host Cell Entry of Ebolaviruses.

Authors - Florian Wrensch
goatWB

Res Vet Sci

Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.

Authors - Xiancheng Zeng
humanWB

Biochem Biophys Res Commun

Differential interferon pathway gene expression patterns in Rhabdomyosarcoma cells during Enterovirus 71 or Coxsackievirus A16 infection.

Authors - Wei Zhang
humanWB,IF

PLoS One

Antiviral Role of IFITM Proteins in African Swine Fever Virus Infection.

Authors - Raquel Muñoz-Moreno
humanWB,FC

Viruses

The Inhibition of HIV-1 Entry Imposed by Interferon Inducible Transmembrane Proteins Is Independent of Co-Receptor Usage.

Authors - Jingyou Yu
  • KD Validated