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  • KD/KO Validated

IFITM2-specific Monoclonal antibody

IFITM2-specific Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3D5F7

Cat no : 66137-1-Ig

Synonyms

DSPA2c, IFITM2



Tested Applications

Positive WB detected inHepG2 cells, recombinant protein
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66137-1-Ig is IFITM2-specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name interferon induced transmembrane protein 2 (1-8D)
Observed molecular weight 15 kDa
GenBank accession numberNM_006435
Gene symbol IFITM2
Gene ID (NCBI) 10581
RRIDAB_2881536
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.

Protocols

Product Specific Protocols
WB protocol for IFITM2-specific antibody 66137-1-IgDownload protocol
FC protocol for IFITM2-specific antibody 66137-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Intrinsic Immunity Shapes Viral Resistance of Stem Cells.

Authors - Xianfang Wu
  • KO Validated
humanWB

Proc Natl Acad Sci U S A

Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.

Authors - Wan-Lin Wu
humanWB

EMBO J

Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells.

Authors - Blandine Monel
humanWB

Proc Natl Acad Sci U S A

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Authors - Guoli Shi
human

Mol Cell Proteomics

HIV-1 Nef changes the proteome of T cells extracellular vesicles depleting IFITMs and other antiviral factors

Authors - Mara E da Silva-Januário
humanWB

PLoS Pathog

p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication.

Authors - Xin Wang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM2 expression in permanent cell lines.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:100 (immunofluorescence), 1:1000 (Western blot)
  • Cell Tissue Type: Permanent cell lines