IFNA14 Recombinant monoclonal antibody

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• IFNA14 Recombinant monoclonal antibody (87828-1-RR)

• 

  WB analysis of Recombinant protein using 87828-1-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 87828-1-RR (IFNA14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 87828-1-RR

  Transfected with IFNA14 HEK-293T cells were subjected to SDS PAGE followed by western blot with 87828-1-RR (IFNA14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  Affinity of 87828-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87828-1-RR against Human IFNA14 were performed. The affinity constant is 2.40 nM.

IFNA14 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87828-1-RR

Clone No.252962C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IFN-alpha-14, Interferon alpha-14, Interferon alpha-H, Interferon lambda-2-H, LeIF H

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Tested Applications

• Positive WB detected in: Recombinant protein, Transfected with IFNA14 HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87828-1-RR targets IFNA14 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5295

  Product name: recombinant human IFNA14 protein

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 24-189 aa of BC074956

  Sequence: CNLSQTHSLNNRRTLMLMAQMRRISPFSCLKDRHDFEFPQEEFDGNQFQKAQAISVLHEMMQQTFNLFSTKNSSAAWDETLLEKFYIELFQQMNDLEACVIQEVGVEETPLMNEDSILAVKKYFQRITLYLMEKKYSPCAWEVVRAEIMRSLSFSTNLQKRLRRKD

• Full Name: interferon, alpha 14
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC074956
• Gene Symbol: IFNA14
• Gene ID (NCBI): 3448
• RRID: AB_3745774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01570
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFNA14 (Interferon α-14) is a type I interferon encoded by a gene located on chromosome 9 (9p21.3). It is mainly produced by macrophages and other cells. By binding to interferon receptors (IFNAR), it initiates signaling pathways such as JAK-STAT, playing a crucial role in antiviral immunity (especially against HIV-1), tumor occurrence, and immune regulation.