IFN-gamma Polyclonal antibody

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  WB analysis using 15365-1-AP

  PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 15365-1-AP

  Recombinant human IFN gamma protein (cat.NO. HZ-1301) were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN gamma antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15365-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15365-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

IFN-gamma Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15365-1-AP

Publications(121)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

IFNG, IFNγ, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN gamma

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Tested Applications

• Positive WB detected in: NK-92 cells, Recombinant protein
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 40 publications below
• IF: See 33 publications below
• ELISA: See 1 publications below
• FC: See 2 publications below

Product Information

15365-1-AP targets IFN-gamma in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFN-gamma fusion protein Ag8321
• Full Name: IFN gamma
• Calculated Molecular Weight: 166 aa, 19 kDa
• GenBank Accession Number: BC070256
• Gene Symbol: IFNG
• Gene ID (NCBI): 3458
• ENSEMBL Gene ID: ENSG00000111537
• RRID: AB_2123037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01579
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Publications

Species	Application	Title
human	IF	Nat Immunol; Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer.; Authors - Di Huang
human	IF	Adv Sci (Weinh); Targeting Deltex E3 Ubiquitin Ligase 2 Inhibits Tumor-associated Neutrophils and Sensitizes Hepatocellular Carcinoma Cells to Immunotherapy; Authors - Xiaoling Wu
	IF	Acta Pharm Sin B; Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy; Authors - Hao Ren
human	IF	Gastroenterology; Interferon-γ-stimulated genes, but not USP18, are expressed in livers of patients with acute hepatitis C.; Authors - Michael T Dill
	IHC	eBioMedicine; Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma; Authors - Tingyu Wen
		J Control Release; Efficient gene delivery by multifunctional star poly (β-amino ester)s into difficult-to-transfect macrophages for M1 polarization; Authors - Tao Bo