IFN-gamma R1 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFN-gamma R1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 10808-1-AP

Synonyms

IFNGR1, IFN-gamma-R-alpha, IFN-gamma-R1, IFN-gamma receptor 1, IFN gamma receptor 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 10808-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10808-1-AP (IFNGR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10808-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10808-1-AP (IFNGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10808-1-AP

  IP result of anti-IFNGR1 (IP:10808-1-AP, 4ug; Detection:10808-1-AP 1:500) with HepG2 cells lysate 1800ug.

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  IP experiment of HepG2 using 10808-1-AP

  IP result of anti-IFNGR1 (IP:10808-1-AP, 4ug; Detection:10808-1-AP 1:300) with HepG2 cells lysate 3000ug.

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  IHC staining of human pancreas cancer using 10808-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10808-1-AP (IFNGR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10808-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10808-1-AP (IFNGR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

10808-1-AP targets IFN-gamma R1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFN-gamma R1 fusion protein Ag1114
• Full Name: interferon gamma receptor 1
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC005333
• Gene Symbol: IFNGR1
• Gene ID (NCBI): 3459
• RRID: AB_2121604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Publications

Species	Application	Title
mouse	IHC	Cancer Discov; Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.; Authors - Wan Du
mouse	WB	Nat Commun; DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.; Authors - Baiwen Chen
human	WB	Dev Cell; Interferon-γ resistance and immune evasion in glioma develop via Notch-regulated co-evolution of malignant and immune cells.; Authors - Elena Parmigiani
human,mouse	WB	Oncogene; Low doses of IFN-γ maintain self-renewal of leukemia stem cells in acute myeloid leukemia; Authors - Xiaoling Xie ; KD Validated
mouse	WB,IHC	Ecotoxicol Environ Saf; Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts; Authors - Jiaqi Tian
human	IHC	J Neuropathol Exp Neurol; Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.; Authors - Rodriguez Fausto J FJ

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