IFNGR1/CD119 Monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 66704-1-Ig (same clone as 66704-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66704-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66704-1-Ig (same clone as 66704-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66704-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 66704-1-Ig (same clone as 66704-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66704-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66704-1-Ig (same clone as 66704-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66704-1-PBS in a different storage buffer formulation.

IFNGR1/CD119 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66704-1-PBS

Clone No.1H3A4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IFNGR1, 1H3A4, CD119, IFN gamma R1, IFN gamma receptor 1

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Product Information

66704-1-PBS targets IFNGR1/CD119 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27085

  Product name: Recombinant human IFNGR1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 189-489 aa of BC005333

  Sequence: TQKEDDCDEIQCQLAIPVSSLNSQYCVSAEGVLHVWGVTTEKSKEVCITIFNSSIKGSLWIPVVAALLLFLVLSLVFICFYIKKINPLKEKSIILPKSLISVVRSATLETKPESKYVSLITSYQPFSLEKEVVCEEPLSPATVPGMHTEDNPGKVEHTEELSSITEVVTTEENIPDVVPGSHLTPIERESSSPLSSNQSEPGSIALNSYHSRNCSESDHSRNGFDTDSSCLESHSSLSDSEFPPNNKGEIKTEGQELITVIKAPTSFGYDKPHVLVDLLVDDSGKESLIGYRPTEDSKEFS

• Full Name: interferon gamma receptor 1
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC005333
• Gene Symbol: IFNGR1
• Gene ID (NCBI): 3459
• RRID: AB_2882056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15260
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interferon-gamma (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)