IFRD2 Polyclonal antibody

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• IFRD2 Polyclonal antibody (26952-1-AP)

• KD/KO Validated

IFRD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26952-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

IFNRP, Interferon-related developmental regulator 2

• 

  WB analysis of HeLa using 26952-1-AP

  WB result of IFRD2 antibody (26952-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFRD2 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HL-60 using 26952-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26952-1-AP (IFRD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 26952-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26952-1-AP (IFRD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 26952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26952-1-AP targets IFRD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25057

  Product name: Recombinant human IFRD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 413-442 aa of BC007265

  Sequence: RFEKHLYNAAAFKARTKARSRVRDKRADIL

• Full Name: interferon-related developmental regulator 2
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC007265
• Gene Symbol: IFRD2
• Gene ID (NCBI): 7866
• RRID: AB_2880700
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12894
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFRD2, or interferon-related developmental regulator 2, is a protein that has been identified as a novel factor capable of translationally inactivating ribosomes. It plays a role in regulating gene expression by directly binding to 80S ribosomes, which precludes the binding of mRNA and P-site tRNA, thereby inhibiting translation

Publications

What published studies show

The IFRD2 Polyclonal antibody (Cat# 26952-1-AP) has 1 citation to date. It appears in Journal of Muscle Research and Cell Motility (IF 2.2, 2024), where it was used for WB and IF in bovine samples. The cited study investigates IFRD2 as a target of miR-2400 regulating myogenic differentiation of bovine skeletal muscle satellite cells via decreased ERK1/2 phosphorylation, placing the research field in muscle biology and myogenic differentiation.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
bovine	WB,IF	J Muscle Res Cell Motil; IFRD2, a target of miR-2400, regulates myogenic differentiation of bovine skeletal muscle satellite cells via decreased phosphorylation of ERK1/2 proteins; Authors - Zhian Gong