IFT172 Polyclonal antibody

IFT172 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28441-1-AP

Synonyms

DKFZp434A163, IFT172, KIAA1179, osm 1, SLB, wim



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue, mouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inARPE-19 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28441-1-AP targets IFT172 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Reactivity mouse, rat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT172 fusion protein Ag28921
Full Name intraflagellar transport 172 homolog (Chlamydomonas)
Calculated Molecular Weight 1749 aa, 198 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC137126
Gene Symbol IFT172
Gene ID (NCBI) 26160
RRIDAB_2881142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IFT172 antibody 28441-1-APDownload protocol
IHC protocol for IFT172 antibody 28441-1-APDownload protocol
IF protocol for IFT172 antibody 28441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols