IFT74 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT74 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27334-1-AP

Synonyms

CCDC2, CMG 1, CMG1, IFT74

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 27334-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27334-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27334-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 27334-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27334-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27334-1-AP (IFT74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse thymus tissue, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 27334-1-AP is IFT74 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT74 fusion protein Ag26385
• Full Name: intraflagellar transport 74 homolog (Chlamydomonas)
• Calculated Molecular Weight: 600 aa, 69 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC107742
• Gene Symbol: IFT74
• Gene ID (NCBI): 80173
• RRID: AB_2880847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Genet Med; Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.; Authors - Minna Luo ; KD Validated
human	WB	Pulm Circ; Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia; Authors - Saejeong Park
human,mouse	IF	Hum Reprod; Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility; Authors - Qi Li
human	WB,IP	Hum Mol Genet; Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations; Authors - Mahmoud R Fassad
human	WB	EMBO J; The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport; Authors - Niels Boegholm ; KO Validated
mouse	WB,CoIP	EMBO Mol Med; IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect; Authors - Jing Dai