IFT81 Monoclonal antibody

IFT81 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Rabbit, canine

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D5A3

Cat no : 67987-1-Ig

Synonyms

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

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Validation Data Gallery

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  WB analysis using 67987-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit brain using 67987-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 67987-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67987-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 67987-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (67987-1-Ig, Clone: 1D5A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rabbit brain tissue, NCCIT cells, hTERT-RPE1 cells, MDCK cells, Human testis tissue, Rat testis tissue, Mouse testis tissue
• Positive IF detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67987-1-Ig targets IFT81 in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, canine samples.

• Tested Reactivity: Human, Rat, Mouse, Rabbit, canine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFT81 fusion protein Ag31629
• Full Name: intraflagellar transport 81 homolog (Chlamydomonas)
• Calculated Molecular Weight: 676 aa, 80 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC029349
• Gene Symbol: IFT81
• Gene ID (NCBI): 28981
• RRID: AB_2918736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.