IFT81 Monoclonal antibody

IFT81 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Rabbit

Applications

WB

Conjugate

Unconjugated

CloneNo.

1D5A3

Cat no : 67987-1-Ig

Synonyms

CDV 1, CDV 1R, CDV1, CDV1R, IFT81



Tested Applications

Positive WB detected inHEK-293 cells, rabbit brain tissue, NCCIT cells, hTERT-RPE1 cells, MDCK cells, Human testis tissue, Rat testis tissue, Mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67987-1-Ig targets IFT81 in WB applications and shows reactivity with Human, Rat, Mouse, Rabbit samples.

Tested Reactivity Human, Rat, Mouse, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFT81 fusion protein Ag31629
Full Name intraflagellar transport 81 homolog (Chlamydomonas)
Calculated molecular weight 676 aa, 80 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC029349
Gene symbol IFT81
Gene ID (NCBI) 28981
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

Protocols

Product Specific Protocols
WB protocol for IFT81 antibody 67987-1-IgDownload protocol
IHC protocol for IFT81 antibody 67987-1-IgDownload protocol
Standard Protocols
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