Validation Data Gallery
|Positive WB detected in
|Human testis, tissue
|Positive IP detected in
|Positive IHC detected in
|human kidney tissue, human heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 60227-1-Ig is IFT88 Fusion Protein expressed in E. coli.
|human, mouse, rat
|Host / Isotype
|Mouse / IgG1
|IFT88 fusion protein Ag4980
|intraflagellar transport 88 homolog (Chlamydomonas)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.
J Cell Physiol
Crosstalk between the COX2-PGE2-EP4 signaling pathway and primary cilia in osteoblasts after mechanical stimulation.
Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.
Front Cell Dev Biol
Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells.
J Cell Physiol
Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.
2-IPMA Ameliorates PM2.5-Induced Inflammation by Promoting Primary Ciliogenesis in RPE Cells
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
k (Verified Customer) (10-26-2023)
I used it with TBST+5% BSA over night for WB analysis.