IGF-II R/IGF2R Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86821-1-RR (same clone as 86821-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86821-1-RR (IGF-II R/IGF2R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86821-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 86821-1-RR (same clone as 86821-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 86821-1-RR (IGF-II R/IGF2R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86821-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86821-1-RR (same clone as 86821-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IGF-II R/IGF2R antibody (86821-1-RR, Clone: 251265D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86821-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86821-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86821-1-RR against Human IGF-II R/IGF2R were performed. The affinity constant is below 1 pM.

IGF-II R/IGF2R Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86821-1-PBS

Clone No.251265D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

IGF2R, 300 kDa mannose 6-phosphate receptor, Cation-independent mannose-6-phosphate receptor, CD222, CI Man 6 P receptor

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Product Information

86821-1-PBS targets IGF-II R/IGF2R in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3488

  Product name: recombinant human IGF-II R/IGF2R protein

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 41-1365 aa of NM_000876.4

  Sequence: QAAPFPELCSYTWEAVDTKNNVLYKINICGSVDIVQCGPSSAVCMHDLKTRTYHSVGDSVLRSATRSLLEFNTTVSCDQQGTNHRVQSSIAFLCGKTLGTPEFVTATECVHYFEWRTTAACKKDIFKANKEVPCYVFDEELRKHDLNPLIKLSGAYLVDDSDPDTSLFINVCRDIDTLRDPGSQLRACPPGTAACLVRGHQAFDVGQPRDGLKLVRKDRLVLSYVREEAGKLDFCDGHSPAVTITFVCPSERREGTIPKLTAKSNCRYEIEWITEYACHRDYLESKTCSLSGEQQDVSIDLTPLAQSGGSSYISDGKEYLFYLNVCGETEIQFCNKKQAAVCQVKKSDTSQVKAAGRYHNQTLRYSDGDLTLIYFGGDECSSGFQRMSVINFECNKTAGNDGKGTPVFTGEVDCTYFFTWDTEYACVKEKEDLLCGATDGKKRYDLSALVRHAEPEQNWEAVDGSQTETEKKHFFINICHRVLQEGKARGCPEDAAVCAVDKNGSKNLGKFISSPMKEKGNIQLSYSDGDDCGHGKKIKTNITLVCKPGDLESAPVLRTSGEGGCFYEFEWHTAAACVLSKTEGENCTVFDSQAGFSFDLSPLTKKNGAYKVETKKYDFYINVCGPVSVSPCQPDSGACQVAKSDEKTWNLGLSNAKLSYYDGMIQLNYRGGTPYNNERHTPRATLITFLCDRDAGVGFPEYQEEDNSTYNFRWYTSYACPEEPLECVVTDPSTLEQYDLSSLAKSEGGLGGNWYAMDNSGEHVTWRKYYINVCRPLNPVPGCNRYASACQMKYEKDQGSFTEVVSISNLGMAKTGPVVEDSGSLLLEYVNGSACTTSDGRQTTYTTRIHLVCSRGRLNSHPIFSLNWECVVSFLWNTEAACPIQTTTDTDQACSIRDPNSGFVFNLNPLNSSQGYNVSGIGKIFMFNVCGTMPVCGTILGKPASGCEAETQTEELKNWKPARPVGIEKSLQLSTEGFITLTYKGPLSAKGTADAFIVRFVCNDDVYSGPLKFLHQDIDSGQGIRNTYFEFETALACVPSPVDCQVTDLAGNEYDLTGLSTVRKPWTAVDTSVDGRKRTFYLSVCNPLPYIPGCQGSAVGSCLVSEGNSWNLGVVQMSPQAAANGSLSIMYVNGDKCGNQRFSTRITFECAQISGSPAFQLQDGCEYVFIWRTVEACPVVRVEGDNCEVKDPRHGNLYDLKPLGLNDTIVSAGEYTYYFRVCGKLSSDVCPTSDKSKVVSSCQEKREPQGFHKVAGLLTQKLTYENGLLKMNFTGGDTCHKVYQRSTAIFFYCDRGTQRPVFLKETSDCSYLFEWRTQYACPPFD

• Full Name: insulin-like growth factor 2 receptor
• Calculated Molecular Weight: 274 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: NM_000876.4
• Gene Symbol: IGF2R
• Gene ID (NCBI): 3482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11717
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4.