IGF2 Recombinant monoclonal antibody

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• IGF2 Recombinant monoclonal antibody (84643-1-RR)

IGF2 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84643-1-RR Copied!

Clone No. 241746D3

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

241746D3, IGF II, IGF-II, Insulin-like growth factor II Ala-25 Del, PP1446

• 

  WB analysis of HuH-7 using 84643-1-RR

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 84643-1-RR (IGF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 84643-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84643-1-RR (IGF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 84643-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84643-1-RR (IGF2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 84643-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84643-1-RR against Human IGF2 were performed. The affinity constant is 0.159 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HuH-7 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84643-1-RR targets IGF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: insulin-like growth factor 2 (somatomedin A)
• Observed Molecular Weight: 20 kDa
• Gene Symbol: IGF2
• Gene ID (NCBI): 3481
• RRID: AB_3672102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01344
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factor 2 (IGF2) is a crucial protein that plays a significant role in regulating growth, particularly during normal fetal development, and is also often dysregulated during tumorigenesis. IGF2 is one of the main ligands of the type 1 IGF receptor (IGF-1R), a tyrosine kinase receptor that transmits signals upon binding with IGF-II. IGF2 is predominantly secreted via the placenta during pregnancy and, after birth, primarily by hepatocytes, with its secretion being independent of growth hormone (GH) secretion, unlike IGF-I.

Publications

What published studies show

The IGF2 Recombinant monoclonal antibody (241746D3), catalog number 84643-1-RR, has 2 citations. These appear in Cell Signal and iScience. The cited studies address ovarian function in premature ovarian insufficiency (PCBP2–IGF2 axis) and clear cell renal cell carcinoma progression (ZBED6–IGF2–PIK3C3 autophagy axis). Both citations used the antibody for Western blot in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Signal; The binding of PCBP2 to IGF2 mRNA restores mitochondrial function in granulosa cells to ameliorate ovarian function in premature ovarian insufficiency mice; Authors - Yuanyuan Chen
human	WB	iScience; ZBED6-IGF2-PIK3C3 autophagy axis drives ccRCC progression: A multi-omics integration study.; Authors - Xiaochen Qi