IGFBP2 Polyclonal antibody

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IGFBP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11065-3-AP Copied!

Publications (26)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

BP 2, BP2, IBP 2, IBP2, IBP-2

• 

  WB analysis of SH-SY5Y using 11065-3-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11065-3-AP (IGFBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of SH-SY5Y using 11065-3-AP

  IP result of anti-IGFBP2 (IP:11065-3-AP, 4ug; Detection:11065-3-AP 1:300) with SH-SY5Y cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 11065-3-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11065-3-AP (IGFBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 11065-3-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11065-3-AP (IGFBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11065-3-AP targets IGFBP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1535

  Product name: Recombinant human IGFBP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC009902

  Sequence: MLPRVGCPALPLPPPPLLPLLPLLLLLLGASGGGGGARAEVLFRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ

• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC009902
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• RRID: AB_2123101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18065
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP2, also known as BP2 and IBP2, is a member of the IGFBP family. It is predominantly expressed in fetal tissues that are highly proliferative, such as the early post-implantation epiblast, the ventricular zone of the rostral neuroepithelium, and has been found to be highly expressed in many cancers (PMID: 31925333). It is a promising protein in non-communicable diseases such as obesity, insulin resistance, metabolic syndrome or type 2 diabetes (PMID: 33498859).

Publications

What published studies show

The anti-IGFBP2 antibody (Cat# 11065-3-AP) has 26 citations across 22 journals, including Nature Communications, Circulation Research, Journal of Clinical Investigation, Cell Death Discovery, and Apoptosis. Research fields span oncology (glioblastoma, lung adenocarcinoma, hepatocellular carcinoma), cardiovascular disease (pulmonary hypertension, myocardial fibrosis), endocrinology (diabetic kidney disease, insulin signaling), reproductive biology (ovarian folliculogenesis, preeclampsia), immunology (lupus nephritis, ulcerative colitis), and developmental biology (ciliary trafficking, angiogenesis).

Automatically generated from the 26 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Nat Commun; Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program.; Authors - Nana Zhang
mouse	WB	Nat Commun; Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.; Authors - Hyunho Kim
mouse	WB	Circ Res; Endothelial FUNDC1 Deficiency Drives Pulmonary Hypertension; Authors - Yandong Pei
human	WB	J Clin Invest; A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.; Authors - Xiaochao Tan ; KD Validated
zebrafish	IHC	Environ Health Perspect; Evaluating the Effects of Neburon Exposure on Ovarian Folliculogenesis Using Zebrafish and Mouse Granulosa Cell Line.; Authors - Chen Tang
human	WB	Cell Death Discov; SVIP reduces IGFBP-2 expression and inhibits glioblastoma progression via stabilizing PTEN; Authors - Zixuan Wang