|Positive WB detected in||SH-SY5Y cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11065-3-AP targets IGFBP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||chicken, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IGFBP2 fusion protein Ag1535|
|Full Name||IGF binding protein 2|
|Calculated molecular weight||35 kDa|
|Observed molecular weight||33-36 kDa|
|GenBank accession number||BC009902|
|Gene ID (NCBI)||3485|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
INS-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degraded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers.
MiR-34b-5p Mediates the Proliferation and Differentiation of Myoblasts by Targeting IGFBP2.
Oxid Med Cell Longev
IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.
Exp Cell Res
HMGA2 contributes to vascular development and sprouting angiogenesis by promoting IGFBP2 production
Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.
J Clin Invest
A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.