IGFBP2 Polyclonal antibody

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  WB analysis using 31248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31248-1-AP (IGFBP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 31248-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31248-1-AP (IGFBP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IGFBP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31248-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

IGF binding protein 2, IBP-2, IBP2, IBP 2, BP2

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Tested Applications

• Positive WB detected in: HEK-293T cells, SH-SY5Y cells, human plasma
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31248-1-AP targets IGFBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_000597.3
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• RRID: AB_3669917
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18065
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP2, also known as BP2 and IBP2, is a member of the IGFBP family. It is predominantly expressed in fetal tissues that are highly proliferative, such as the early post-implantation epiblast, the ventricular zone of the rostral neuroepithelium, and has been found to be highly expressed in many cancers (PMID: 31925333). It is a promising protein in non-communicable diseases such as obesity, insulin resistance, metabolic syndrome or type 2 diabetes (PMID: 33498859).