IGFBP6 Monoclonal antibody

IGFBP6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1D12B3

Cat no : 67567-1-Ig

Synonyms

IBP 6, IBP6, IGF binding protein 6, IGFBP 6, IGFBP6

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Validation Data Gallery

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  WB analysis of U-87 MG using 67567-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 67567-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67567-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67567-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-87 MG cells, human testis tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

67567-1-Ig targets IGFBP6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, monkey
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IGFBP6 fusion protein Ag30050
• Full Name: insulin-like growth factor binding protein 6
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC011708
• Gene Symbol: IGFBP6
• Gene ID (NCBI): 3489
• RRID: AB_2882781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factor (IGF) binding protein (IGFBP6), a 240 amino acid protein, contains an IGFBP N-terminal domain and a thyroglobulin type-1 domain. It modulates the activity of IGF and shows independent effects of IGF, such as growth inhibition and apoptosis. It can decrease the proliferation and survival of cancer cells such as lung cancer cells and naso-pharyngeal cancer cells. IGFBP-6 is distinctive for its 50-fold higher binding affinity for IGF-II over IGF-I and this specificity makes it an attractive potential therapeutic candidate for IGF-II-dependent pediatric malignancies such as rhabdomyosarcoma (RMS). In addition, it was found that IGFBP6 can promote the migration of RMS cells in an IGF-independent manner, and MAPK pathways were involved in this process. Further study reported that IGFBP6 is one of most highly expressed proteins in varicose vein tissues and is involved in the proliferation of vascular smooth muscle cells (VSMCs), which may provide insights into the underlying pathogenesis of varicose vein.

Publications

Species	Application	Title
human	IHC	Neurol Res; The clinical characteristics and prognostic value of IGFBP6 in glioma.; Authors - Zhitao Zong
monkey	IHC	Front Cell Dev Biol; Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone; Authors - Hiroki Hagizawa