IGFBP7 Polyclonal antibody

IGFBP7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 19961-1-AP

Synonyms

FSTL2, IBP 7, IGF binding protein 7, IGFBP 7, IGFBP 7v, IGFBP rP1, IGFBP7, IGFBPRP1, MAC25, MAC25 protein, PGI2 stimulating factor, PSF, TAF, Tumor derived adhesion factor

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Validation Data Gallery

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  WB analysis using 19961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19961-1-AP (IGFBP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19961-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19961-1-AP (IGFBP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse appendix using 19961-1-AP

  IP result of anti-IGFBP7 (IP:19961-1-AP, 4ug; Detection:19961-1-AP 1:300) with mouse appendix tissue lysate 6000ug.

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  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells, SKOV-3 cells, THP-1 cells
• Positive IP detected in: mouse appendix tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

19961-1-AP targets IGFBP7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: insulin-like growth factor binding protein 7
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_001553
• Gene Symbol: IGFBP7
• Gene ID (NCBI): 3490
• RRID: AB_2878630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP7, also named as MAC25, PSF, IBP-7 and TAF, binds IGF-I and IGF-II with a relatively low affinity. IGFBP7 stimulates prostacyclin (PGI2) production. IGFBP7 stimulates cell adhesion.

Publications

Species	Application	Title
mouse	IHC	J Am Soc Nephrol; Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI.; Authors - Ali C M Johnson
human	IHC,WB	Cancer Lett; IGFBP7-AS1 is a p53-responsive long noncoding RNA downregulated by Epstein-Barr virus that contributes to viral tumorigenesis.; Authors - Wei Dang
mouse	IF	Hum Genet; MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract.; Authors - Sandeep Aryal
human	WB	Oral Dis; FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization; Authors - Xiangrui Ma
rat	IHC	J Cell Biochem; IGFBP7 contributes to epithelial-mesenchymal transition of HPAEpiC cells in response to radiation; Authors - Yazhen Zhong
	WB	J Cell Mol Med; The Circ_35953 induced by the NF-κB mediated the septic AKI via targeting miR-7219-5p/HOOK3 and IGFBP7 axis; Authors - Yuqing Feng