Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21465-1-AP targets IGSF21 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15062 Product name: Recombinant human IGSF21 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 117-467 aa of BC007618 Sequence: HVGIYDRATREKVVLASGNIFLNVMAPPTSIEVVAADTPAPFSRYQAQNFTLVCIVSGGKPAPMVYFKRDGEPIDAVPLSEPPAASSGPLQDSRPFRSLLHRDLDDTKMQKSLSLLDAENRGGRPYTERPSRGLTPDPNILLQPTTENIPETVVSREFPRWVHSAEPTYFLRHSRTPSSDGTVEVRALLTWTLNPQIDNEALFSCEVKHPALSMPMQAEVTLVAPKGPKIVMTPSRARVGDTVRILVHGFQNEVFPEPMFTWTRVGSRLLDGSAEFDGKELVLERVPAELNGSMYRCTAQNPLGSTDTHTRLIVFENPNIPRGTEDSNGSIGPTGARLTLVLALTVILELT Predict reactive species |
| Full Name | immunoglobin superfamily, member 21 |
| Calculated Molecular Weight | 467 aa, 52 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | BC007618 |
| Gene Symbol | IGSF21 |
| Gene ID (NCBI) | 84966 |
| RRID | AB_10734429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96ID5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGSF21 (Immunoglobulin superfamily member 21) is a GPI-anchored protein that contains two immunoglobulin-like domains (Ig-like 1 and Ig-like 2). The Ig-like 1 domain of IGSF21 is indispensable for synaptogenic activity whereas its Ig-like 2 domain is secondarily responsible for the activity. It has been reported that IGSF21 selectively regulates inhibitory presynaptic differentiation through interacting with presynaptic neurexin2α and plays a crucial role in synaptic inhibition in the brain. (PMID: 28864826)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGSF21 antibody 21465-1-AP | Download protocol |
| WB protocol for IGSF21 antibody 21465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-IgSF21 antibody (Cat# 21465-1-AP) has 5 total citations published in Nature Communications, Frontiers in Immunology (×2), EMBO Reports, and Frontiers in Molecular Neuroscience. Research fields span neuroscience (inhibitory synapse organization, nuclear transport pathology in schizophrenia), immunology (IgLON5 autoantibodies and knockout behavioral studies), and neurodegeneration. Applications include WB, IHC, and CoIP across mouse and human species.
| Species | Application | Title |
|---|---|---|
Nat Commun IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.
| ||
Front Immunol IgLON5 deficiency produces behavioral alterations in a knockout mouse model | ||
EMBO Rep Schizophrenia-related Xpo7 haploinsufficiency leads to behavioral and nuclear transport pathologies | ||
Front Mol Neurosci Structural and functional characterization of the IgSF21-neurexin2α complex and its related signaling pathways in the regulation of inhibitory synapse organization |
