IGSF8/CD316 Polyclonal antibody

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• IGSF8/CD316 Polyclonal antibody (33948-1-AP)

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  WB analysis using 33948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33948-1-AP (CD316/IGSF8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 33948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33948-1-AP (IGSF8/CD316 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 33948-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33948-1-AP (IGSF8/CD316 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Neuro-2a using 33948-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using IGSF8/CD316 antibody (33948-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IGSF8/CD316 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 33948-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD316, CD81 partner 3, Ewi2, EWI-2, Glu-Trp-Ile EWI motif-containing protein 2

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Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33948-1-AP targets IGSF8/CD316 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg6320

  Product name: Recombinant mouse CD316/IGSF8 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 26-577 aa of AF439263.1

  Sequence: RQVHVPRGPLYRVAGTAVSISCNVSDYEGPAQQDFEWFMYRPEAPATSLGIVSTKDSQFSYAVFGPRVASGDLQVQRLKGDSVVLKIARLQAQDSGFYECYTPSTDTQYLGNYSAKVELRVLPDELQVSAAPPGPRGRQAATSPSRLTVHEGQELALGCLAQTKTKKHTHLSVSFGRAIPEAPVGRATLQEVVGLRSDMAVEAGAPYAERLASGELRLSKEGTDRYRMVVGGAQAGDSGTYHCTAAEWIQDPDGSWVQVAEKRAVLAHVDVQTLSSQLAVTVGPGERRIGPGEPLELLCNVSGALPPPGRHAAYSVGWEMAPAGAPGPGRLVAQLDTEGIGSLGPGYEDRHIAMEKVASRTYRLRLEAARPADAGTYRCLAKAYVRGSGTRLREAASARSRPLPVHVREEGVVLEAVAWLAGGTVYRGETASLLCNISVRGGPPGLRLAASWWVERPEEGELSSGPAQLVGGVGQDGVAELGVRPGGGPVSVELVGPRSHRLRLHGLGPEDEGIYHCAPSAWVQHADYSWYQAGSARSGPVTVYPYTHAVDT

• Full Name: immunoglobulin superfamily, member 8
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: AF439263.1
• Gene Symbol: Igsf8
• Gene ID (NCBI): 140559
• RRID: AB_3743119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8R366
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)