IHCeasy Alpha Actinin Ready-To-Use IHC Kit
Alpha Actinin Ready-to-use reagent kit for IHC.
Cat no : KHC0088
Synonyms
actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1
Validation Data Gallery
Product Information
KHC0088 is a ready-to-use IHC kit for staining of Alpha Actinin (ACTN1). The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy Alpha Actinin Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1 |
