IHCeasy CFLAR/FLIP Ready-To-Use IHC Kit

CFLAR/FLIP Ready-to-use reagent kit for IHC.

Reactivity

Human

Sample Type

FFPE tissue

Cat no : KHC0610

Synonyms

c FLIP, c FLIPL, c FLIPR, c FLIPS, CASH, CASP8AP1, Caspase eight related protein, Caspase homolog, Casper, CFLAR, CFLAR/FLIP, CLARP, FLAME, FLAME 1, FLAME1, FLIP, I FLICE, Inhibitor of FLICE, MRIT, Usurpin



Product Information

KHC0610 is a ready-to-use IHC kit for staining of CFLAR/FLIP. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy CFLAR/FLIP Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

CFLAR, also named as CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6 mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms c FLIP, c FLIPL, c FLIPR, c FLIPS, CASH, CASP8AP1, Caspase eight related protein, Caspase homolog, Casper, CFLAR, CFLAR/FLIP, CLARP, FLAME, FLAME 1, FLAME1, FLIP, I FLICE, Inhibitor of FLICE, MRIT, Usurpin