IHCeasy Human IgG4 Ready-To-Use IHC Kit

Human IgG4 Ready-to-use reagent kit for IHC.

Reactivity

Human

Sample Type

FFPE tissue

Cat no : KHC0074

Synonyms

Human IgG4, IgG4, IGHG4



Product Information

KHC0074 is a ready-to-use IHC kit for staining of Human IgG4. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy Human IgG4 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Mouse Monoclonal
Secondary antibody type Polymer-HRP-Goat anti-Mouse
Reactivity Human

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

Serum IgG4 is a subtype of IgG, and its levels are frequently elevated and particularly high in autoimmune pancreatitis (AIP) as well as other disease. It's now a common concept that IgG4 related disease (IgG4-RD) is a systemic immune-mediated fibro-inflammatory condition often involving several organ systems, especially pancreas and salivary glands. A serum IgG4 cut off level of 135mg/mL was considered a reliable marker predictive of IgG4-RD. IgG4 positive plasma cell infiltration is also ubiquitous in IgG4-RD. (PMID: 33274670)

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms Human IgG4, IgG4, IGHG4