IHCeasy ISCU Ready-To-Use IHC Kit

ISCU Ready-to-use reagent kit for IHC.


Human, Mouse, Rat

Sample Type

FFPE tissue

Cat no : KHC1259


2310020H20Rik, HML, hnifU, ISCU, ISCU2, ISU2, NIFU, NifU like protein, NIFUN

Product Information

KHC1259 is a ready-to-use IHC kit for staining of ISCU. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy ISCU Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

Iron-sulfur (Fe-S) clusters are required for the functions of mitochondrial aconitase, mammalian iron regulatory protein 1, and many other proteins in multiple subcellular compartments. Mitochondrial and cytosolic isoforms of the human Fe-S cluster scaffold protein, iron-sulfur cluster assembly enzyme (ISCU), is involved in activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins. Defects in ISCU gene are the cause of myopathy with exercise intolerance Swedish type (MEIS), which is characterized by lifelong severe exercise intolerance. Two ISCU isoforms ISCU1 (cytosolic) and ISCU2 (mitochondrial), were produced by alternative splicing, and ISCU2 is considered the canonical isoform1. ISCU2 protein is rapidly processed upon maturation.


Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms 2310020H20Rik, HML, hnifU, ISCU, ISCU2, ISU2, NIFU, NifU like protein, NIFUN