IHCeasy SURVIVIN Ready-To-Use IHC Kit

SURVIVIN Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat no : KHC0646

Synonyms

API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, EPR 1, IAP4, SURVIVIN



Product Information

KHC0646 is a ready-to-use IHC kit for staining of SURVIVIN. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy SURVIVIN Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, EPR 1, IAP4, SURVIVIN