HELIOS Monoclonal antibody

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  WB analysis of Jurkat using 68558-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MOLT-4 using 68558-1-Ig

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (IKZF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 68558-1-Ig

  IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug.

HELIOS Monoclonal Antibody for WB, IP, ELISA

Cat No. 68558-1-Ig

Clone No.1F1F10

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, ELISA

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68558-1-Ig targets HELIOS in WB, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4357

  Product name: Recombinant human IKZF2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 227-526 aa of BC028936

  Sequence: NNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH

• Full Name: IKAROS family zinc finger 2 (Helios)
• Calculated Molecular Weight: 500 aa, 57 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC028936
• Gene Symbol: HELIOS
• Gene ID (NCBI): 22807
• RRID: AB_3085260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UKS7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).