IKZF3 Polyclonal antibody

IKZF3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13561-1-AP

Synonyms

AIO, AIOLOS, IKZF3, Zinc finger protein Aiolos, ZNFN1A3

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Validation Data Gallery

• 

  WB analysis of Jurkat using 13561-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13561-1-AP (IKZF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13561-1-AP targets IKZF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IKZF3 fusion protein Ag4434
• Full Name: IKAROS family zinc finger 3 (Aiolos)
• Calculated Molecular Weight: 509 aa, 58 kDa
• Observed Molecular Weight: 58-70 kDa
• GenBank Accession Number: BC032707
• Gene Symbol: IKZF3
• Gene ID (NCBI): 22806
• RRID: AB_2124968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function.; Authors - Liang Zhou
human	IHC	Leuk Lymphoma; PMID: 29718735; Authors - Konstantinos Dimopoulos