Tested Applications
| Positive WB detected in | PMA and LPS treated THP-1 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84411-5-RR targets IL-1 alpha in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Product name: HumanKine® recombinant human IL-1 alpha protein Source: -derived, Tag: Sequence: Predict reactive species |
| Full Name | interleukin 1, alpha |
| Calculated Molecular Weight | 271 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC013142 |
| Gene Symbol | IL-1 alpha |
| Gene ID (NCBI) | 3552 |
| RRID | AB_3671940 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01583 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-1 alpha antibody 84411-5-RR | Download protocol |
| WB protocol for IL-1 alpha antibody 84411-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-1 alpha Recombinant monoclonal antibody (241752F1), catalog number 84411-5-RR, has 1 total citation. It appears in Translational Cancer Research (2026), in a study investigating microplastics- and nanoplastics-related gene signatures for prognosis prediction in pancreatic ductal adenocarcinoma, with functional validation of interleukin-1 alpha. The associated research fields include pancreatic cancer, environmental toxicology (microplastics/nanoplastics), and cytokine-mediated inflammation.
| Species | Application | Title |
|---|---|---|
Transl Cancer Res Microplastics and nanoplastics-related genes signature predicts prognosis in pancreatic ductal adenocarcinoma and functional validation of interleukin 1 alpha. |
