IL-10 Monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP50035-1

  Sandwich ELISA standard curve of MP50035-1, IL-10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69018-1-PBS. Detection antibody: 69018-2-PBS. Standard: HZ-1145. Range: 15.6-1000 pg/mL.

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  Sample test of MP50035-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 day or 5 days. For the PBMC supernatant cultured for 1 day, the mean IL-10 concentration was determined to be 431.4 pg/mL in unstimulated PBMC supernatant, 1,534.2 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean IL-10 concentration was determined to be 225.9 pg/mL in unstimulated PBMC supernatant, 634.8 pg/mL in PHA stimulated PBMC supernatant.

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  Affinity and Kinetic Characterization of 69018-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 69018-2-Ig against Human IL-10 were performed. The affinity constant is 0.426 nM.

IL-10 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 69018-2-PBS

Clone No.7B8G3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

NeutraKine® IL-10, IL-10,IL10, 7B8G3, CSIF, IL 10

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Product Information

69018-2-PBS targets IL-10 as part of a matched antibody pair:

MP50035-1: 69018-1-PBS capture and 69018-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: interleukin 10
• Gene Symbol: IL-10
• Gene ID (NCBI): 3586
• Conjugate: Unconjugated
• Purification Method: Protein A purification
• UNIPROT ID: P22301
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.