rat IL-10 Recombinant antibody

IL-10 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A4

Cat no : 82793-16-RR

Synonyms

Il10, IL-10, IL10X, interleukin 10

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Validation Data Gallery

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  WB analysis of Recombinant protein using 82793-16-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 82793-16-RR (IL-10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82793-16-RR targets IL-10 in WB, ELISA applications and shows reactivity with rat, mouse samples.

• Tested Reactivity: rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 10
• Calculated Molecular Weight: 20kd
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_012854
• Gene Symbol: Il10
• Gene ID (NCBI): 25325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.