Tested Applications
| Positive FC detected in | anti-CD3/CD28 treated human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98149-1-RR targets IL-12RB1/CD212 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant Protein Predict reactive species |
| Full Name | interleukin 12 receptor, beta 1 |
| Calculated Molecular Weight | 662 aa, 73 kDa |
| GenBank Accession Number | BC029121 |
| Gene Symbol | IL12RB1 |
| Gene ID (NCBI) | 3594 |
| RRID | AB_3672294 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P42701 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
Interleukin-12 receptor subunit beta-1 (IL-12RB1), also named as CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. IL-12RB1 localizes in the membrane and functions as an interleukin receptor which binds IL-12 with low affinity and is involved in IL-12 transduction. Associated with IL-12RB2 it forms a functional, high affinity receptor for IL-12. It can also associate with IL23R to form the interleukin-23 receptor which functions in IL-23 signal transduction probably through activation of the Jak-Stat signaling cascade.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-12RB1/CD212 antibody 98149-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
