Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24173-1-AP targets IL-1F6/IL-36 alpha in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21487 Product name: Recombinant human IL-1F6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-158 aa of BC107043 Sequence: MEKALKIDTPQQGSIQDINHRVWVLQDQTLIAVPRKDRMSPVTIALISCRHVETLEKDRGNPIYLGLNGLNLCLMCAKVGDQPTLQLKEKDIMDLYNQPEPVKSFLFYHSQSGRNSTFESVAFPGWFIAVSSEGGCPLILTQELGKANTTDFGLTMLF Predict reactive species |
| Full Name | interleukin 1 family, member 6 (epsilon) |
| Calculated Molecular Weight | 158 aa, 18 kDa |
| GenBank Accession Number | BC107043 |
| Gene Symbol | IL-1F6/IL-36 alpha |
| Gene ID (NCBI) | 27179 |
| RRID | AB_2879446 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHA7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL-1F6, also known as IL-36α (IL36A), is a pro-inflammatory member of the IL-1 cytokine family that is primarily expressed in skin and lymphoid tissues, with lower levels detected in fetal brain, trachea, stomach, and intestine. It is uniquely produced by monocytes, B cells, and T cells among the novel IL-1 family ligands, and its expression is markedly elevated in psoriatic plaques. Functionally, IL-36α signals through a heterodimeric receptor complex comprising IL-1Rrp2 (IL-36R) and IL-1RAcP, thereby activating NF-κB and MAPK signaling pathways to induce the production of inflammatory mediators such as IL-6, IL-8, and antimicrobial peptides including BD-2 and BD-3. As a key regulator of both innate and adaptive immunity, IL-36α plays a critical role in epithelial barrier defense and tissue inflammation, and its dysregulation has been implicated in inflammatory skin diseases, kidney disorders, and fibrotic conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-1F6/IL-36 alpha antibody 24173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-1F6/IL-36 alpha Polyclonal antibody (Cat# 24173-1-AP) has 3 citations published in Experimental Dermatology, Frontiers in Oncology, and Journal of Cutaneous Pathology. All citations used IHC in human samples. Research fields span dermatology (severe dermatitis, palmoplantar psoriasis vs. eczema differential diagnosis) and oncology (IL-36-related gene prognosis in gastric cancer).
| Species | Application | Title |
|---|---|---|
Exp Dermatol Clinical Responses and Transcriptomic Analysis of Spesolimab in a Girl With Severe Dermatitis, Multiple Allergies and Metabolic Wasting Syndrome | ||
J Cutan Pathol IL-36α and IL-36γ expressions in the differential diagnosis of palmoplantar psoriasis and palmoplantar eczema: A retrospective histopathologic and immunohistochemical study. |
