IL-1RA/IL-1RN Recombinant antibody

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  WB analysis using 85672-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85672-3-RR (IL-1RA/IL-1RN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 85672-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 85672-3-RR (IL-1RA/IL-1RN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 85672-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 85672-3-RR (IL-1RA/IL-1RN antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-1RA/IL-1RN Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85672-3-RR

Clone No.242317D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

IL1 inhibitor, IL1RA, IL-1ra, Il1rn, IL-1RN

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Tested Applications

• Positive WB detected in: C2C12 cells, mouse skin tissues
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85672-3-RR targets IL-1RA/IL-1RN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-1RA/IL-1RN fusion protein Eg3206
• Full Name: interleukin 1 receptor antagonist
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: NM_022194.2
• Gene Symbol: Il1rn
• Gene ID (NCBI): 60582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25086
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.