IL-23 p19 Recombinant monoclonal antibody

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  WB analysis using 85947-2-RR

  Recombinant human IL-23 protein (HZ-1254) and Recombinant human IL-12B protein (HZ-13211) were subjected to SDS PAGE followed by western blot with 85947-2-RR (IL-23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 85947-2-RR

  Human IL-23 Recombinant protein (CataNo. Eg1304) were subjected to SDS PAGE followed by western blot with 85947-2-RR (IL-23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IL-23 p19 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85947-2-RR

Clone No.250414G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IL23A, IL 23, IL 23 A, IL 23 subunit alpha, IL 23A

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85947-2-RR targets IL-23 p19 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human IL-23 protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: interleukin 23, alpha subunit p19
• Gene Symbol: IL23A
• Gene ID (NCBI): 51561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NPF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin 23 (IL-23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of Il-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL-6. The main biological effects of IL-23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages.