Rat IL-4 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP01293-4

  Sandwich ELISA standard curve of MP01293-4, Rat IL-4 Recombinant Matched Antibody Pair - PBS only. 84422-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1113. 84422-5-PBS was Biotin conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01293-4

  Serum of eight individual healthy human donors was measured. Seven measured less than the lowest rat IL-4 standard, 39.1 pg/mL. One sample read 18.7 pg/mL.

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  Sample test of MP01293-4

  One rat thymus was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 5 μg/mL of PHA and 50 ng/mL of PMA for 7 days. An aliquot of the cell culture supernatant was removed, assayed for rat IL-4 and measured 98.5 pg/mL.

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  Affinity and Kinetic Characterization of 84422-3-PBS

  Biolayer interferometry (BLl) kinetic assays of 84422-3-PBS against Rat IL-4 were performed. The affinity constant is below 1 pM.

IL-4 antibodies are versatile tools for investigating IL-4’s roles in immunity, allergy, autoimmunity, and cancer, supporting a wide range of molecular, cellular, and functional assays. Proteintech has an extensive validated IL-4 antibodies for Sandwich ELISA, Indirect ELISA, Sample test.

Cat No. 84422-3-PBS

Clone No.241249E11

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1, Il4

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Product Information

84422-3-PBS targets IL-4 as part of a matched antibody pair:

MP01293-4: 84422-3-PBS capture and 84422-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-4 fusion protein Eg1113
• Full Name: interleukin 4
• Calculated Molecular Weight: 16kDa
• GenBank Accession Number: NM_201270.1
• Gene Symbol: IL-4
• Gene ID (NCBI): 287287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20096
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.