IL-4 Recombinant monoclonal antibody

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• IL-4 Recombinant monoclonal antibody (84422-5-RR)

• 

  WB analysis of Recombinant protein using 84422-5-RR

  Rat IL-4 recombinant protein (Cat No. Eg113) was subjected to SDS PAGE followed by western blot with 84422-5-RR (IL-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IL-4 antibodies are versatile tools for investigating IL-4’s roles in immunity, allergy, autoimmunity, and cancer, supporting a wide range of molecular, cellular, and functional assays. Proteintech has an extensive range of validated IL-4 antibodies antibodies for WB, IHC, IF-P, ELISA and more.

Cat No. 84422-5-RR

Clone No.241249H7

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IF, ELISA

Synonyms

Il4, BSF-1, B-cell stimulatory factor 1, B-cell IGG differentiation factor, B-cell growth factor 1

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

84422-5-RR targets IL-4 in WB, IF, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: interleukin 4
• Calculated Molecular Weight: 16kDa
• GenBank Accession Number: NM_201270.1
• Gene Symbol: IL-4
• Gene ID (NCBI): 287287
• RRID: AB_3743557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20096
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in regulating immune responses and mediating type-2 immune reactions. It is primarily produced by T-helper 2 (Th2) cells, mast cells, eosinophils, basophils, and other immune cells. IL-4 exerts its effects by binding to type I and type II receptors, initiating a signaling cascade that involves the phosphorylation of STAT6 and the transcription of IL-4-responsive genes. This pathway is essential for the regulation of immune cell functions and the maintenance of immune homeostasis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IF	Bioact Mater; Engineered nerve guide conduit enhances peripheral nerve regeneration by modulating the microenvironment through macrophage-triggered cascade reactions.; Authors - Xindi Yang
rat	WB	Food Sci Nutr; Targeting to Intensify M2 Macrophage Polarization Ameliorate Podocyte Lipid Accumulation and Damage by Tea Polyphenols via Activating SIRT1 in the Aged Model Rats With DKD.; Authors - Shuangzhi Chen