IL-4 Recombinant antibody

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  WB analysis using 85179-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85179-5-RR (IL-4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 85179-5-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using IL-4 antibody (85179-5-RR, Clone: 242736G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IL-4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85179-5-RR

Clone No.242736G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Il 4, Il4, interleukin 4

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Tested Applications

• Positive WB detected in: C2C12 cells, EL-4 cells, THP-1 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85179-5-RR targets IL-4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 4
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC027514
• Gene Symbol: IL-4
• Gene ID (NCBI): 16189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.