IL-4 Recombinant monoclonal antibody

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• IL-4 Recombinant monoclonal antibody (85179-5-RR)

IL-4 antibodies are versatile tools for investigating IL-4’s roles in immunity, allergy, autoimmunity, and cancer, supporting a wide range of molecular, cellular, and functional assays. Proteintech has an extensive range of validated IL-4 antibodies antibodies for WB, IHC, IF-P, ELISA and more.

Cat No. 85179-5-RR Copied!

Clone No. 242736G10

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Il 4, Il4, interleukin 4

• 

  WB analysis using 85179-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85179-5-RR (IL-4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 85179-5-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using IL-4 antibody (85179-5-RR, Clone: 242736G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C2C12 cells, EL-4 cells, THP-1 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85179-5-RR targets IL-4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: interleukin 4
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC027514
• Gene Symbol: IL-4
• Gene ID (NCBI): 16189
• RRID: AB_3744078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The IL-4 Recombinant monoclonal antibody (242736G10), catalog number 85179-5-RR, has 5 total citations. These appear in European Journal of Pharmacology, International Immunopharmacology, Toxicology, Journal of Nutritional Biochemistry, and Biochemical and Biophysical Research Communications. The research spans neuroinflammation and depression, ferroptosis-related renal fibrosis, gut microbiome–driven atopic dermatitis, and macrophage-mediated bone repair.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Eur J Pharmacol; Essential roles of IL-4 signaling in the prophylactic effect of gardiquimod on chronic stress-induced depression-like behaviors and neuroinflammatory responses.; Authors - Nan Liu
		Int Immunopharmacol; TREM2 regulates neuroinflammation by SYK-dependent inhibition of BTK activation to improve perioperative neurocognitive dysfunction.; Authors - Kaiyun Zhang
mouse	WB,IF	Toxicology; Chronic exposure to polystyrene microplastics induces renal fibrosis via ferroptosis; Authors - Runyang Hong
mouse	WB	J Nutr Biochem; High-fat diet exacerbates atopic dermatitis through alterations in the gut microbiome.; Authors - Keito Tabata
mouse	WB	Biochem Biophys Res Commun; PLCγ1 knockdown in BMSCs enhances bone repair via IL-4-JAK2-STAT3-mediated reparative macrophage polarization.; Authors - Fen Wang