IL-5 Polyclonal antibody

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• IL-5 Polyclonal antibody (30416-1-AP)

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  WB analysis using 30416-1-AP

  Human IL-5 Recombinant protein (Cat No. Eg2406) was subjected to SDS PAGE followed by western blot with 30416-1-AP (IL-5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IL-5 Polyclonal Antibody for WB, ELISA

Cat No. 30416-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IL5, B-cell differentiation factor I, IL 5, Interleukin 5, Interleukin-5

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30416-1-AP targets IL-5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0380

  Product name: Recombinant Human IL-5 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 20-134 aa of BC066282

  Sequence: IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

• Full Name: interleukin 5 (colony-stimulating factor, eosinophil)
• Calculated Molecular Weight: 134 aa, 15 kDa
• GenBank Accession Number: BC066282
• Gene Symbol: IL5
• Gene ID (NCBI): 3567
• RRID: AB_3742341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05113
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-5 (IL-5) is a pleiotropic cytokine primarily produced by activated T helper 2 (Th2) cells, mast cells, and group 2 innate lymphoid cells (ILC2s). It plays a critical role in the regulation of eosinophil proliferation, differentiation, and activation, making it a key mediator in allergic inflammation, parasitic infections, and certain autoimmune diseases.