IL-5RA/CD125 Recombinant monoclonal antibody

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• IL-5RA/CD125 Recombinant monoclonal antibody (84948-5-RR)

• 

  WB analysis of human peripheral blood platelets using 84948-5-RR

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 84948-5-RR (IL-5 R alpha/CD125 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MC/9 using 84948-5-RR

  Immunofluorescent analysis of (4% PFA) fixed MC/9 cells using IL-5RA/CD125 antibody (84948-5-RR, Clone: 242234H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IL-5RA/CD125 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84948-5-RR

Clone No.242234H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IL5RA, 242234H8, CD125, HSIL5R3, IL 5 receptor subunit alpha

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Tested Applications

• Positive WB detected in: human peripheral blood platelets
• Positive IF/ICC detected in: MC/9 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84948-5-RR targets IL-5RA/CD125 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1665

  Product name: Recombinant Human IL-5RA/CD125protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 21-335 aa of NM_175726.4

  Sequence: DLLPDEKISLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNVNLEYQVKINAPKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHAPPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVNGSSKHSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQPIYVGNDE

• Full Name: interleukin 5 receptor, alpha
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_175726.4
• Gene Symbol: IL-5RA/CD125
• Gene ID (NCBI): 3568
• RRID: AB_3743874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01344-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-5 receptor subunit alpha (IL-5RA), also known as CD125, is a type I transmembrane protein that belongs to the type I cytokine receptor family. IL-5RA is a critical component of the high-affinity IL-5 receptor complex, IL-5R, which consists of the alpha chain (IL-5RA) that specifically binds to IL-5 and the common beta chain (CD131) shared with receptors for IL-3 and GM-CSF (PMID: 1833065; 7872157). IL-5/IL-5RA signaling axis is crucial for the differentiation, proliferation, recruitment, and activation of eosinophils, and is also involved in pathological eosinophilia (PMID: 34638583).