IL-5RA/CD125 Recombinant monoclonal antibody

• 

  WB analysis of human peripheral blood platelets using 84948-5-RR

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 84948-5-RR (IL-5 R alpha/CD125 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IL-5RA/CD125 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84948-5-RR

Clone No.242234H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IL5RA, IL 5R subunit alpha, IL 5R alpha, IL 5 receptor subunit alpha, HSIL5R3

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Tested Applications

• Positive WB detected in: human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84948-5-RR targets IL-5RA/CD125 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: interleukin 5 receptor, alpha
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_175726.4
• Gene Symbol: IL-5RA/CD125
• Gene ID (NCBI): 3568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01344-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-5 receptor subunit alpha (IL-5RA), also known as CD125, is a type I transmembrane protein that belongs to the type I cytokine receptor family. IL-5RA is a critical component of the high-affinity IL-5 receptor complex, IL-5R, which consists of the alpha chain (IL-5RA) that specifically binds to IL-5 and the common beta chain (CD131) shared with receptors for IL-3 and GM-CSF (PMID: 1833065; 7872157). IL-5/IL-5RA signaling axis is crucial for the differentiation, proliferation, recruitment, and activation of eosinophils, and is also involved in pathological eosinophilia (PMID: 34638583).