IL-7 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• IL-7 Recombinant monoclonal antibody (84165-5-RR)

• 

  WB analysis using 84165-5-RR

  Human IL-7 recombinant protein was subjected to SDS PAGE followed by western blot with 84165-5-RR (IL-7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 84165-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84165-5-RR (IL-7 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-7 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84165-5-RR

Clone No.252659C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

IL7, IL 7, interleukin 7, Interleukin-7

---

Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84165-5-RR targets IL-7 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human IL-7 protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: interleukin 7
• Gene Symbol: IL-7
• Gene ID (NCBI): 3574
• RRID: AB_3743527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13232
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-7 (IL-7) is a non-redundant, pleiotropic cytokine fundamental to the development, homeostasis, and function of the immune system. It is primarily produced by stromal cells in the bone marrow and thymus, as well as by epithelial cells in peripheral lymphoid organs. IL-7 signals through a heterodimeric receptor consisting of the private IL-7 receptor alpha chain (IL-7Rα/CD127) and the common gamma chain (γc), activating key pathways such as JAK/STAT, PI3K/Akt, and MAPK to promote lymphocyte survival, proliferation, and metabolic fitness. Its critical role is underscored by the severe lymphopenia and immunodeficiency observed in both IL-7- and IL-7R-deficient humans and mice.